Partial freezing of rat livers extends preservation time by 5-fold.

3c ), although differences between the lactate levels at the discrete time points did not reach statistical significance.

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While all livers that were stored in the frozen state at −10 °C for 1 day showed a decreasing trend of tissue adenylate energy charge (Fig. 2e ), statistically significant decreases were only observed for GLY (0.064 ± 0.039 at 2 h) and EG (0.088 ± 0.065 at 2 h), as compared to control livers (0.24 ± 0.073 at 2 h; p = 0.0033 and p = 0.0097, respectively).

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While an increasing trend was observed in cfDNA and HSP70 collected from partially frozen versus control livers, this mostly did not reach statistical significance, except for an increase in HSP70 between 1 day frozen and Clinical Oxy.

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