Switchable Control of Scaffold Protein Activity via Engineered Phosphoregulated Autoinhibition.

A clear trend in the autoinhibition level was observed for the three scaffolds (from 35-fold decrease for dT14-3-3_biPKA to 10-fold for dT14-3-3_biPKB), which can be attributed to the differences in the binding affinity of the attached peptide motifs. 33 , 43 Binding studies with the high-affinity biExoS peptide provide an additional stringent test, given its binding affinity in the low nanomolar range.

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