Sofosbuvir induces gene expression for promoting cell proliferation and migration of hepatocellular carcinoma cells.

In another recent study, Ogawa et al., found that the annual incidence of the development of HCC within the first two years was higher for HCV patients treated with SOF than for those without, but did not reach statistical significance [ 45 ].

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Real-time PCR After the identification of highly significant target genes, the primers for real-time PCR were designed with Primer Express 3.0 (Applied Biosystems, Foster City, CA, USA).

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