moderately significantp -value < 0.05
On the other hand, when we used the ACh stimulation protocol ( Figure 2 e–h), the average distance between DBH centroids and the nearest α3β4 nAChR centroids (184.0 ± 50.2 nm) was not only significantly lower than the average one when compared with a random simulation for α3β4 nAChR structures (605.7 ± 9.7 nm; p -value < 0.0001, Figure 2 i), but also showed a moderately significant average distance reduction to that obtained for the K + stimulated assays ( p -value < 0.05).