Mixed metal zero-mode guides (ZMWs) for tunable fluorescence enhancement.

There is a clear trend of larger enhancement with increasing Au content with the exception that 100Al shows much better fluorescence intensity than expected which could arise from increase in excitation rate.

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There is also a decreasing trend in fluorescence intensity of single ATTO 590 molecules from 75Al to 100Au that signifies a red shift in the compatibility of ZMWs with increasing Au content.

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