From the up-regulated ones, we have determined the mRNA expression levels of Nebulette, Flavin containing dimethylaniline monooxygenase 2, and Ankyrin repeat domain 1 and while all of them showed a trend for increasing in mice subjected to PAB, these results were not significant ( Supplementary material online , Figure S1b ).
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The adult heart requires baseline expression of the transcription factor Hand2 to withstand right ventricular pressure overload.
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