qRT-PCR performed on HEMF primary cultures used for RNA-Seq also showed a trend in IL-6 mRNA in three out of four primary cultures, and a significant increase in one primary culture (HEMF 1).
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Inflammatory and Proliferative Pathway Activation in Human Esophageal Myofibroblasts Treated with Acidic Bile Salts.
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There was an increasing trend in the mRNA expression of IL-6 in three out of four primary HEMF cultures, with a statistically significant difference in primary culture established from HEMF 1 ( Figure 2 B).