Although there was a notable trend of more hydrophobic labels having bigger signal intensities in one laboratory, we observed no such trend in the other laboratory.
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In a pursuit of optimal glycan fluorescent label for negative MS mode for high-throughput N-glycan analysis.
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The sentences
In Lab1, we detected a clear trend towards better ionization efficiency with more hydrophobic labels, as expected.
Nevertheless, an obvious trend of signal drop among structurally similar labels such as 2AA-3AA-4AA and 32ANA-62ANA, possibly explained by their different pKa values and resulting dissociation differences, indicates that presence of negative charge on the label actually has unfavourable effect on glycan ionization in negative mode MS.