Multi-spectral immunofluorescence evaluation of the myeloid, T cell, and natural killer cell tumor immune microenvironment in chordoma may guide immunotherapeutic strategies.

Although proliferating CD4 + T cells (median 1.24 and 1.00 cells per mm 2 in tumor and stroma, respectively, p = 0.888) and PD1 + CD4 + T cells (median 1.05 and 0.46 cells per mm 2 in tumor and stroma, respectively, p = 0.180) were slightly more abundant in the tumor parenchyma, these small differences failed to reach statistical significance.

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