Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage.

During the overwintering period in 2021, the changes in the proline content of BLF treatment were similar to those in 2020, which also showed a trend of first increasing and then decreasing, while the proline content of CK was basically stable ( Figure 6D ).

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With cold acclimation, the EL-LT50 showed a decreasing trend in the early overwintering period, demonstrating that the freezing tolerance of plants gradually increased, reaching maximum value in January.

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