ALDH3B1 protects interfollicular epidermal cells against lipid peroxidation via the NRF2 pathway.

In addition, we cultured IFE cells with different holotransferrin concentrations (0 μg/ml, 1 μg/ml, 10 μg/ml, 100 μg/ml, and 1 mg/ml) for 48 h, and after protein extraction, protein blotting analysis confirmed that ALDH3B1 expression showed an elevated trend (Fig. 3c , d ).

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The results showed a trend of decreasing intracellular lipid peroxidation level with increasing holotransferrin concentration (Fig. 2i , j ).

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