Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling.

ISME Commun · 2021 · PMC9723555 · PMID 37938227

Europe PMC DOI

hedged sentence

0.2270

closest p · 4.5× alpha

0.2270

boldest claim

The sentences

did not reach statistical significancep = 0.227not close (p > 0.1)

On average, the proportion of spurious OTUs in both the mock communities and samples from gnotobiotic mice was slightly lower after removing singletons, although this did not reach statistical significance (50.8 vs. 64.3%, p = 0.227; 57.5% vs. 65.7%; p = 0.70, pairwise comparison by t -test, including Benjamini–Hochberg correction following ANOVA).

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