Gene expression underlying parenting and being parented shows limited plasticity in response to different ambient temperatures.

Females interacting with larvae and larvae interacting with parents shared more differentially expressed genes in common ( n = 115) than parenting males and parented larvae ( n = 28), although this did not reach statistical significance ( p = .059) and neither comparison showed global signatures of concordance of gene expression (Figure 2g,h ). 3.2.3.

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While overlap of the most highly significant genes between two sets lends itself to intuitive interpretation, use of fixed thresholds can be overly stringent and lead to false negatives.

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