Genome-scale top-down strategy to generate viable genome-reduced phages.

The T4 mutants showed a trend similar to that of the T7 mutants (Figure 4A ), in that the ability of the phages to kill the host cells became weaker (the killing time increased from 80 min to 250 min) as the number of transfers increased; however, a high percentage (25%) of the mutants showed an increased ability to kill cells (killing time <115 min) compared with WT T4 (Figure 4A ).

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