a strong trendP = 0.0505
There was a strong trend (Newman–Keuls test, P = 0.0505) towards higher II at S4 (pre-adult) compared to S3 in females, both sexes had significantly lower II at S5 (48 h in fw) than S4 and lastly females but not males had significantly lower (Newman–Keuls test, P < 0.05) II at S6 compared to S5.