We further validated the enrichment of C7_CXCL13 cells in CD8 + T cells of hematogenous route samples via multiplex immunohistochemistry (IHC) staining of markers (CXCL13, TIM3 and CD8), which showed a consistent result with our single-cell data, although the results failed to reach statistical significance, probably due to the small sample size (Fig. 5h ; Supplementary Fig. 6h ).
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Evolutionary route of nasopharyngeal carcinoma metastasis and its clinical significance.
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