Optimization and Technical Considerations for the Dye-Exclusion Protocol Used to Assess Blood-Brain Barrier Integrity in Adult <i>Drosophila melanogaster</i>.

Int J Mol Sci · 2023 · PMC9916281 · PMID 36768206

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Fixation conditions involving head removal (with or without the proboscis) both showed a trend toward an injury-induced increase in TMRD infiltration, but the elevated baseline fluorescence of the no-hit conditions may have masked the impact of injury on the barrier disruption.

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