Pixel-by-pixel autofluorescence corrected FRET in fluorescence microscopy improves accuracy for samples with spatially varied autofluorescence to signal ratio.

Sci Rep · 2023 · PMC9941493 · PMID 36804608

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a clear trendno p-value reported

The AVG corrected FRET efficiencies followed the trends in signal/autofluorescence values in the FRET and acceptor channels while the non-corrected values did not follow a clear trend.

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