highly significantp < 0.0001
These results were further analyzed using Design-Expert 12.0.3 software to obtain quadratic polynomial regression equations (as shown below) for the extraction of sorghum 3-DAs. Y = 0.2760 + 0.0419 A − 0.0206 B + 0.0128 C + 0.0136 AB + 0.0098 AC − 0.0119 BC − 0.0109 A 2 − 0.0422 B 2 − 0.0456 C 2 ANOVA was performed on the above regression model, and the results are presented in Table 3 ; these results indicated that the linear correlation of the regression model was highly significant ( p < 0.0001).