Significances were declared at P ≤ 0.05, and 0.05 < P ≤ 0.10 were considered as a significant trend. 16S amplicon sequencing of the fecal microbial community The total DNA was extracted from fecal samples using the E.Z.N.A.®Stool DNA Kit (#D4015, Omega, Inc., USA).
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Gut microbiome is linked to functions of peripheral immune cells in transition cows during excessive lipolysis.
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