The expression difference between VIGS and control plants was highly significant in a two-tailed Student's t -test of 2 ΔCt values (VIGS 0.1 ± 0.05; Untreated 0.53 ± 0.01, p -value = 7 × 10 −5 , Figure 3Bb ).
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The ANOVA indicated a highly significant GxY interaction for GZn [ p < 0.0001, F (374, 563) = 1.78] and a non-significant GxY for GFe [ p = 0.06, F (374, 561) = 1.15].
A strong and highly significant knock-down of expression ( P < 0.0001, n = 12) was detected 3 days post-agroinfiltration ( Figure 5 ).
As expected, the correlation between SNPP value of hybrid F 1 and the total GCA effect of parents was highly significant ( r = 0.82; P < 0.0001).
Variance analyses showed that the effects of population, photoperiod treatment and population × photoperiod treatment interaction on days to bolting, days to flowering, rosette leaf number, and flower stem length were highly significant ( P ≤ 0.0001; Supplementary Table S2 ).
wheat landraces Ethiopian tetraploid wheat association analysis yellow rust resistance 90 K wheat SNP array Highly significant differences were observed among accessions and accessions by environments ( P < 0.0001) in ANOVA analyses for stripe rust response in the field tests, but no significant difference was detected among environments.
The measure of population differentiation, F ST , among the subgroups was 0.15 highly significant at P < 0.0001.
The association between phaseolin type and cluster membership was highly significant and nearly complete (χ 2 = 174.72; df = 1; P < 0.0001; Cramér's V = 0.93).
The difference between the distribution center of the two subpopulations was highly significant [ F (6, 85) = 11.246, P < 0.0001].
When pooled together, the two groups of lines “low RAS/RT ratio” (L8, L82, and L44) and “high RAS/RT ratio” (L92, L12, L118, L14, L57, and L39) were clearly separated in terms of average RAS/RT ratio, the difference being highly significant (respectively 9.15 < 19.35 g/g; Student t -test p < 0.0001).
Discussion Highly significant differences ( p ≤ 0.0001) were observed between the accessions for all of the studied traits (Table 1 ).
The analysis of variance (one-way ANOVA) detected highly significant differences among RILs for all traits in each environment ( p < 0.0001, Appendix C ), which indicates that genetic factors explain a large fraction of the observed phenotypic variability.
14 C-glyphosate Uptake and Translocation Assays The differences in foliar uptake of 14 C-glyphosate between the resistant hairy beggarticks populations compared to the S population were highly significant ( P < 0.0001; DF = 2; n = 60) ( Figure 3A ).
In the same region, we identified several markers near-significant ( p < 0.0001) for crown rust reaction and severity and one marker significant for days to anthesis (GMI_ES03_c7453_413).
Nonetheless, a highly significant and negative correlation between free-ABA and total anthocyanins was observed for both irrigation treatments ( r = −0.76, p ≤ 0.0001) in the 2 years.
Accessions with different polymorphisms at bPb-9668 and bPb-0003 showed highly significant differences in salinity tolerance ( P < 0.0001, Figure 5A ; Supplementary Table S2 ).
The analysis of variance for stem rust reaction showed highly significant differences ( P < 0.0001) among accessions included in the whole collection, the durum sub-sample and Q2 group.
Data analyses for soil C, N, and K showed a highly significant interaction between year and soil type (C: P < 0.0001; N: P < 0.0001; K: P = 0.0009).
As shown in Figure 6 , analysis of the core W-box motif (TTGACY) in promoters of these genes by POBO (Materials and Methods) shows that enrichment of this motif is highly significant ( p -value < 0.0001) compared to randomly selected promoters from all Arabidopsis genes.
The highly significant slopes ( P < 0.0001) between the linear relationships ranged from 0.7 to 1.1, with the weakest relationships apparent for Q. robur (average slope of 0.8 for all methods), likely caused the relatively low amount of detect vessels and its variance between methods.
Percent total protein decreased in maize seed endosperms transformed with the soybean ferritin transgene The mean percentage total protein differences between PCR positive and negative maize seed endosperm samples were highly significant ( P < 0.0001) (Table 4 ).
The regressions implicating altitude and latitude of provenances were highly significant ( P < 0.0001), resulting in R 2 ranging between 0.21 and 0.51 (Table 2 ).
Influence of light treatment on plant height was highly significant ( p < 0.0001).
Genetic differentiation among the sub-clusters was highly significant ( P < 0.0001).
All main effects such as genotype, trial, and their interaction, were highly significant ( p < 0.0001) for all studied traits.