1G , plasma insulin levels measured from samples collected 10 min into the last IPGTT experiments displayed a near-significant tendency to be reduced acutely but increased at 6 hours after exendin-4 treatment for β-arrestin 2 KO versus control mice when normalized to their corresponding vehicle values (fig.
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Divergent acute versus prolonged pharmacological GLP-1R responses in adult β cell-specific β-arrestin 2 knockout mice.
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The sentences
However, this was reversed overnight, with this temporal trajectory conveniently expressed as “overnight over acute,” an effect significant for islets from mice on chow diet and close to significance for HFHS diet ( Fig. 4, E and F , and fig.
Parallel experiments, depicted in the same figure, were performed using the GIPR agonist GIP, where we observed the same trend, which, in agreement with our in vivo results suggesting a lesser dependence of GIPR on β-arrestin 2, did not quite reach statistical significance.
S1E), although we did detect a small tendency toward improved insulin sensitivity in β cell–specific β-arrestin 2 KO versus control animals, as assessed by intraperitoneal insulin tolerance test (IPITT), that did not reach statistical significance (fig.
S1I), resulting in a highly significant increase in prolonged over acute levels in the β-arrestin 2 KO animals ( Fig. 1I ).