Utilization of commercial collagens for preparing well-differentiated human beta cells for confocal microscopy.

Cells on C6745 showed a trend toward lower insulin content when corrected for the number of cells seeded compared with those on C5533 ( Figure 4A , Supplementary Figure 3A ) that may reflect a difference in number of beta cells attached to the glass surface and/or insulin content per beta cell.

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The insulin granule number per cell trended from highest to lowest in C5533, Col V, and C6745 at low glucose ( Figures 4D, E ) but did not reach statistical significance.

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