Optimization of Tissue Digestion Methods for Characterization of Photoaged Skin by Single Cell RNA Sequencing Reveals Preferential Enrichment of T Cell Subsets.

Due to variability between patient samples, although there is a strong trend demonstrating the substantially better viability of non-frozen cells, it is not statistically significant (unpaired t -test, p = 0.3264).

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Therefore, while there is not a statistically significant difference in the number of total cells per gram of tissue isolated between the sequential and simultaneous digestion methods (paired t -test, p = 0.1158), there is a clear trend of more cells isolated using the sequential method (six of seven samples tested) and consistently better viability for all seven skin samples directly compared.

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