1 A, the proportion of CD4 + CD25 high CD127 low Tregs demonstrated a trend of continuously increasing at 4, 11, and 14 day post coculture and then showed a trend of decreasing at 18 day post coculture (Fig. 1 A).
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CD4<sup>+</sup>CD25<sup>+</sup> regulatory T cells ex vivo generated from autologous naïve CD4<sup>+</sup> T cells suppress EAE progression.
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Although higher numbers of CCN3 + Olig2 + was observed in the lumbar segments of Treg-treated mice than in those of either sham-treated mice or WT mice, the difference among these three groups did not reach statistical significance (Fig. 4 C).