Neutrophil Diversity (Immature, Aged, and Low-Density Neutrophils) and Functional Plasticity: Possible Impacts of Iron Overload in β-Thalassemia.

As such, a positive correlation (red color) was demonstrated only in (i) the chemotaxis of immature neutrophils (unstimulating and PMA stimulation), (ii) ROS and NETs of aged unstimulated neutrophils, and (iii) the apoptosis of aged LPS-stimulated neutrophils; however, these parameters did not reach statistical significance ( Figure 2 G).

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Overall, immature (CD16− CD62L+) and aged (CD16+ CD62L−) neutrophils showed a trend toward negative correlation in most of the functional parameters (most of the heat map shows a blue color demonstrating a lower r 2 ), and only a few pairs of correlation reached significant values ( Figure 2 G).

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