Artificial insemination and laying cycle influence adaptive immune cell numbers and localization in the reproductive tract of turkey breeder hens.

Differences were considered significant when p-value ≤ 0.05 and tended towards significance when 0.05 < p -value ≤ 0.1.

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CD3+ T cell counts in the SSTs, UVJ, and vagina There was a significantly higher number of CD3+ T cells in the SSTs at the start of lay compared to the end of lay ( n = 0.008), with a trend toward significance from peak lay to end of lay ( n = 0.07), regardless of insemination treatment ( Fig. 3a ).

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Within the end-of-lay group, the number of CD3+ T cells showed a trend toward significance ( n = 0.11), being higher in the semen-inseminated hens compared to the sham-inseminated hens ( Fig. 3c ).

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