Characterisation of a Japanese Encephalitis virus genotype 4 isolate from the 2022 Australian outbreak.

For JEV NSW/22 , viremia at day 3 post infection was higher on average but this did not reach statistical significance (Fig. 1C ).

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hBOs infected with JEV isolates shrank in circumference by ~5 to 15% by 11 dpi, although when compared with uninfected organoids, this only approached significance for JEV NSW/22 (Fig. 7D ; Supplementary Fig. 13B, C ). hBOs infected with MVEV TC123130 , IMOJEV and YFV 17D infected hBO did not shrink significantly when compared with uninfected controls, although the MVEV TC123130 data suggested a marginal reduction in circumference (Fig. 7C ; Supplementary Fig. 13C ).

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