A systematic screening assay identifies efficient small guide RNAs for CRISPR activation.

For the Tfeb promoter, a 1.5-fold increase in gene activation was observed in cultures transfected with sgRNA 4 + 3; however, this increase did not reach statistical significance ( Figure 5A ).

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However, a near-significant increase in Tfeb activity was observed when sgRNA 4 + 3 were co-transfected, along with the previously noted increase in Sirt1 expression upon co-transfection of sgRNA 1 + 3 or sgRNA 1 + 4.

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