A splice donor in <i>E6</i> influences keratinocyte immortalization by beta-HPV49.

E8-/SD217 mt genomes expressed significantly lower E6 levels (2.4-fold) than E8- genomes, whereas SD217 mt genomes showed a trend toward increased E6 expression in comparison to the wt as expected upon inactivation of SD217 ( Fig. 6A ).

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SD217/SA3281 levels were also lower in SD217 mt-transfected cells compared with the wt, but this did not reach statistical significance, most likely due to the overall lower transcript levels from wt and SD217 mt genomes.

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