Astrocyte-secreted factors modulate synaptic protein synthesis as revealed by puromycin labeling of isolated synaptosomes.

We therefore exposed neurons to astrocyte-conditioned medium (CM) for 3 days and observed a strong trend toward an increase of puromycin puncta in Syn-Homer synaptosomes ( p = 0.05; Figure 4A ii ), which became significant in post- ( Figure 4A iii ) and presynaptic compartments ( Figure 4A iv ).

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Interestingly, we found that upon direct treatment with astrocyte-CM, Syn-Homer synapses showed a trend toward increasing locally synthesized Rpl26 when compared to synaptosomes co-incubated with anisomycin ( Figures 6B i,ii , p = 0.09).

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