Immunotoxicity assessment of multiwalled carbon nanotubes following whole-body inhalation exposure for 30 and 90 days in B6C3F1/N mice and 30 days in HSD:Harlan Sprague Dawley SD<sup>®</sup> rats.

A decrease was also observed when expressed as AFC/spleen but did not reach statistical significance ( Figure 3F ).

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In addition, there was a positive trend in NK cell numbers with all exposure levels being significantly elevated.

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Shirley’s test was used if a significant trend was observed ( Shirley, 1977 ) and Dunn’s test was used if the trend was not significant ( Dunn, 1964 ).

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There was also a decreasing trend in the relative lymphocyte counts in exposed rats.

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