Establishment and molecular characterization of novel luminal A and luminal B canine mammary cancer cell lines for comparative oncology.

However, due to the high SD observed in the REM134 measurements, these differences did not reach statistical significance when comparing the novel cell lines to the REM134 cell line ( Figure 6 ). mRNA expression profiles of CMC genes The relative expression levels of four common cancer-related genes ( EGFR, TP53, Bcl-2 , and PTEN ) and three EMT transcription factors ( E-cadherin, N-cadherin , and SNAIL ) were evaluated and compared between two novel cell lines using RT-qPCR.

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The results demonstrated that the CMGT_071020 cell line had significantly greater migratory ability than the CMGT_180321 cell line and showed a trend toward higher migratory ability than the REM134 cell line.

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