Acute aerobic exercise intensity does not modulate pain potentially due to differences in fitness levels and sex effects: results from a pharmacological fMRI study.

We have conducted an additional LMER model where we have excluded the subjects with the highest and lowest FTP values (sub-28 with 3.19 W/kg and sub-06 with 0.76 W/kg, respectively.) The LMER still yields a significant main effect of fitness level (β = 6.82, CI [1.25, 11.65], SE = 3.18, t(34) = 2.14, P = 0.039; Author response image 4) and a positive correlation between the difference ratings and fitness level approaching significance (r = 0.32, P = 0.057).

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We also investigated the contrast heat NLX > heat SAL where activation in the right antIns showed a trend at whole-brain correction (MNI xyz : 46, 8, 6, T = 5.70, p corr-WB = 0.08; Figure 5—figure supplement 2 ).

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