Salivary hormonal analysis as a tool for skeletal maturity assessment.

As shown in Table 6 , for CVMI, the p-values were highly significant in females ( p = 0.00061) and males ( p = 0.00371).

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However, these trends did not reach statistical significance in ANOVA ( p > 0.05) (Table 6 ). 17β-Estradiol showed divergent patterns between sexes.

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For Cortisol, females exhibited an increasing trend from CS1 (72.5 pg/mL) to CS5 (101.1 pg/mL), with a slight decline at CS6 (96.1 pg/mL).

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However, ANOVA results indicated no significant differences within each sex ( p = 0.609 for females, p = 0.064 for males), though the latter approached significance (Table 2 ).

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