Sub-chronic stress exerts partially distinct behavioral and epigenetic effects in male and female mice.

Methylation of IKKβ did not differ significantly between the groups, as the main effects of stress [ F (1,18) = 3.572, p = 0.078] and sex [ F (1,18) = 0.094, p = 0.764] and their interaction [ F (1,18) = 0.075, p = 0.788] failed to reach statistical significance ( Figure 5I ).

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Regarding the promoter region of PDYN in the NAc, stress increased PDYN promoter methylation [ F (1,18) = 4.738, p = 0.046], but the effect of sex [F (1,18) = 1.323, p = 0.268] and the stress-by-sex interaction [ F (1,18) = 0.012, p = 0.915] did not reach statistical significance ( Figure 4K ).

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There were no group differences in methylation of the PDYN promoter ( Figure 4E ), as the effects of stress [ F (1, 22) = 2.199, p = 0.155], sex [ F (1, 22) = 2.329, p = 0.143], and their interaction [F (1, 22) = 2.727, p = 0.115] all failed to reach significance.

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