The ABR P1 latency showed a trend toward prolongation, with a significant difference observed at certain frequencies (two-way ANOVA, interaction: F (5, 95) = 2.72, p = 0.024; Šidák post hoc test: p = 0.0028, Cohen d = 2.58 at 8.00 kHz, p = 0.0089, Cohen d = 1.58 at 11.33 kHz, p = 0.019, Cohen d = 1.18 at 16.00 kHz, p < 0.0001, Cohen d = 2.74 at 22.65 kHz, p < 0.0001, Cohen d = 2.46 at 32.00 kHz; Fig 2D ).
Although none of the turns showed a statistically significant difference in the SM area ( Fig 5C ), we observed a trend toward significance in the apical turn with a large effect size (two-way ANOVA, interaction: F (2, 24) = 0.72, p = 0.50; Šidák post hoc test: p = 0.052, Cohen d = 2.05).
Although p=0.052 is not significant, it is a trend towards significance.
However, at several frequencies, ABR P1 latency did not reach statistical significance, likely due to the reduced statistical power resulting from the smaller sample size.