A design-of-experiments strategy for engineering 3D topographical features in osteosarcoma modelling.

Although nominally significant differences in metabolite levels were observed between the doxorubicin-treated groups, none remained significant after correction for multiple testing, with all false discovery rates (FDR) exceeding 0.05 ( Fig.

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While the higher mean aggregate count was statistically significant for MG-63 cells cultured on smooth microparticles relative to the 3D control, the corresponding aggregate count increase in U2OS cells cultured on smooth microparticles did not reach statistical significance.

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