Headspace SPME GC-MS Analysis of Urinary Volatile Organic Compounds (VOCs) for Classification Under Sample-Limited Conditions.

Furthermore, combining NaCl addition with an acidic environment (pH 3) resulted in a trend towards an even greater increase in extraction efficiency, suggesting a synergistic effect between acidity and the salting-out phenomenon ( Figure 2 B), although this effect did not reach statistical significance.

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The addition of NaCl showed a trend toward further increasing extraction, suggesting a possible synergistic effect with acidification, although this was not statistically significant.

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