Remodelling of P-bodies and the cytoskeleton by <i>Orthohantavirus puumalaense</i> (Puumala virus).

For P-bodies, the mean distance did not follow a clear trend, but N protein was found at increasing distances from the nucleus over time, with a maximum at 168 h.

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Interestingly, we observed a highly significant increase in the number of P-body spots in infected cells compared with non-infected cells and mock samples, which again points to a remodelling of cellular structures upon PUUV infection ( Fig. 3b ).

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Whereas S-mRNA and M-mRNA distance to the cell centre significantly increased over time, L -mRNA showed a trend towards shorter distances, albeit not significantly.

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