TMAO Supplementation to High-Carbohydrate Diet Reprogrammed Hepatic Metabolism and Intestinal Microbiota to Improve Liver Health and Disease Resistance of <i>Micropterus salmoides</i>.

Furthermore, TMAO supplementation changed the structure of the intestinal microbiota and particularly the intestinal content of Romboutsia , an important probiotic that can effectively utilize different kinds of dietary carbohydrate, showed an increasing trend with the increased TMAO supplementation levels.

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Results showed that fish fed diets supplemented with TMAO exhibited a trend toward improved SGR and WGR with the prolonged rearing period, though the improvement did not reach statistical significance, indicating that TMAO supplementation may exhibit a significant growth-promoting role in largemouth bass with a longer rearing period.

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The relative abundances of Staphylococcus , Weissella , Bacillus , Lactobacillus , Geomicrobium , Acinetobacter and Escherichia-Shigella showed a trend of decreasing with the increased TMAO level.

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