Uncovering Hidden Phenotypes in NEX-Cre Mice: Behavioral and Cellular Alterations Demand Re-Evaluation of a Widely Used Transgenic Line.

Although the median fluid intake of NEX (+/+) and NEX (+/Cre) mice was slightly elevated, these differences did not reach statistical significance.

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However, increased locomotor activity was observed in NEX (Cre/Cre) mice compared to C57BL/6J mice, with NEX (+/+) and NEX (+/Cre) mice showing a slight trend toward increased locomotor activity (Figure 1K ).

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In the BLA, no significant group differences were observed, although NEX (Cre/Cre) mice showed a trend toward lower spine density (Figure 4J ).

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