Notably, SP-CRA exhibited a graded pattern of highly significant cytokine production, for all five assessed cytokines (IL-2: 1552-fold, IL-6: 38-fold, IL-10: 76-fold, IFN-γ: 1261-fold, and TNF-α: 425-fold) following anti-CD28- SA treatment.
← all excerpts
Evaluation of a reference antibody panel for prediction of cytokine release in humanised mouse models <i>in vivo</i>.
4
—
—
The sentences
Notably, the levels observed for all tested cytokines, except for IL-2, did not reach statistical significance ( Figures 4B–D ).
Notably, the pattern sustained kinetics of anti-CD3-induced TNF-α as compared to the early and transient TNF-α response elicited by anti-CD52 appears to exhibit an overall trend like the human clinical response though these responses are still temporally distinct from those observed clinically.
Overall, while both unsupervised clustering and conventional gating showed similar trends, unsupervised clustering provided improved visualisation of treatment-control differences that may have biological implications but failed to reach statistical significance in conventional analyses ( Figures 6 , 7 ). 3.4 Secretome analysis of mice engrafted with HSCs or PBMCs reveals both shared and distinct molecular signatures Cellular profile and cytokine analyses (discussed above), showed that each Ab in the reference panel, 19/156, elicited distinct patterns of cell depletion and CR.