We then validated our findings using western-blotting for AATC, which showed a trend for decreased levels in SNCA 3x already at a basal level ( p = 0.2775), with a higher tendency noted upon the addition of TNF ( p = 0.1870) (Fig. 5H ), in line with the proteomics finding.
Although FAO dependency did not differ significantly across genotypes or treatments, a mild trend toward increased FAO reliance was observed in TNF-treated SNCA 3x ENLs under CSB6, potentially reflecting partial restoration of metabolic flexibility 74 .
This cluster was consistently detected across biological replicates, although the overall increase in mitoSOX fluorescence per cell type did not reach statistical significance in bulk quantification.