Depletion of BIS in astrocytes aggravates reactive gliosis following photothrombotic brain injury.

Quantitative analysis revealed that GFAP expression was significantly increased by 19% in infarct border astrocytes of BIS-aKO mice compared with controls, whereas GFAP expression in peri-infarct astrocytes was increased by 15%, but this change did not reach statistical significance ( Fig. 3K ).

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The mitochondrial number normalized to cytoplasmic area showed a trend toward reduction (~0.7-fold), although this change was not statistically significant ( Fig. 4O ).

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