PCR profiling of tumor-associated microorganisms in tissue of primary epithelial malignant tumors of colorectal origin: associations with key clinicopathological characteristics.

When comparing within-panel profile distances between the G2–G3 group and G1, no clear separation of group centroids was observed for the primary Aitchison metric (CLR-Euclidean) (PERMANOVA: p=0.094; R2=0.009), whereas Bray–Curtis distances showed a nominally significant difference with a small effect size (PERMANOVA: p=0.030; R2=0.022).

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Pairwise comparisons identified differences only between stage IV and the other stages, suggesting a mild trend toward lower Total bacterial load at stage IV; this trend was likewise not supported after multiple-testing correction.

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Total bacterial load showed a trend toward decreasing values with increasing tumor stage (T1 to T4), with median log10 values of 10.78 [10.65–10.81], 10.17 [9.98–10.60], 10.30 [9.85–10.60], and 10.00 [9.30–10.30], respectively.

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