Spatiotemporal dynamics and ossification of podoplanin-positive cells in the developing femur.

BV/TV on the ventral side showed a decreasing trend but did not reach statistical significance (WT: 32.7 ± 5.9%, n = 4; KO: 25.6 ± 7.0%, n = 6; p = 0.0667, Mann-Whitney U test, Fig. 7 D ventral).

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In the trabecular bone region, quantitative analysis did not reach statistical significance; however, we observed a strong trend toward a marked decrease in PDPN-positive cell numbers with ages (density of PDPN-positive cells in the trabecular bone, 0 w.o: 716 ± 93.0, 1 w.o: 217 ± 148, 2 w.o: 78.2 ± 42.7, 3 w.o: 52.6 ± 33.9, respectively, n = 3, p = 0.0552 in 0 w.o vs. 3 w.o, Kruskal-Wallis test followed by the Dunn’s multiple comparisons test, Fig. 4 E).

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