Cognition at the core of metabolic syndrome: linking metabolic load to behavioural impairment in a longitudinal high-fat diet rat model.

Two-way ANOVA revealed significant effects in time (F (2,16) = 6.456, p = 0.0088), nutritional treatment (F (1,10) = 20.53, p = 0.0011), and a tendency towards significance in their interaction (F (2,16) = 3.169, p = 0.0693) ( Fig. 2 E).

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As for time spent at the centre of the open field maze, HFD rats displayed a decreasing trend over time compared to NPD controls, although no statistical significance is observed via two-way ANOVA in terms of time (F (2,18) = 36.42, p < 0.0001), nutritional treatment (F (1,10) = 22.57, p = 0.0008) and their interaction (F (2,18) = 0.2372, p = 0.7913) ( Supplementary Figure 3 ).

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dROMS showed a trend-level negative association with RI% ret 1H ( Table 3 A, Supplementary ).

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The indirect effect of body weight on RI% ret 1H via dROMS was negative and close to significance ( Table 3 B, Supplementary ), suggesting a potential mediation pattern.

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