Effects of the Temperature and <i>Limosilactobacillus fermentum</i> Co-Inoculation on the Expression of AFB1-Synthesis Genes and the Level of Toxin Produced by <i>Aspergillus flavus</i> Zt41 in Corn Silage.

The 21-day value also showed a significantly higher expression at the mRNA level compared to day 16, but it did not reach statistical significance ( p = 0.109) ( Figure 1 E). 2.2. mRNA Expression Analysis of A. flavus Zt41 Genes Responsible for Toxin Production at 30 °C and the Effect of L. fermentum Co-Inoculation At 30 °C, aflR expression remained stable over time and was unaffected by L. fermentum co-inoculation ( Figure 2 A).

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An increasing trend in AFB1 concentration was observed with rising temperature, although the differences did not reach statistical significance due to high inter-replicate variability.

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fermentum co-inoculation did not produce statistically significant differences, a clear trend toward reduced expression was observed ( Figure 3 E). 2.4.

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