Although a formal test for a genetic interaction between MKT1-30G and SAL1-nfs failed to reach statistical significance (ANOVA, interaction P -value = 0.095), these results suggest epistasis between these two alleles such that their joint effect exceeds that of their individual effects.
The growth rate of distal isolates began to climb for ORFs just telomeric of MKT1 (first without reaching statistical significance, e.g., at SNN1 ), became different from the wild-type isolates at nominal significance for MKT1 and END3 , reached Bonferroni-significance for SAL1 , and continued to be nominally significant for 12 (10 of which with Bonferroni-significance) of the remaining 13 targeted ORFs between SAL1 and the centromere.